In article <30qsvjEnq0 at uni-erlangen.de>,
Robert Slany <mfb102 at cd4680fs.rrze.uni-erlangen.de> wrote:
>you can save the PMSF, it will decay anyway within minutes in aqueous solution>I assume you included PMSF during your purification procedure, so all serine
>(PMSF sensitive) proteases should be inactivated irreversibly.
>My proteins were more stable when stored in higher concentrations (>5mg/ml)
the protein was obtained after thrombin cleavage of a gst-fusion
protein. although the thrombin was removed by a mono-q step, i added the
pmsf just to inhibit any residual thrombin left in the preparation and
avoid non-specific degradation of my protein over time.
bipin k. dalmia the other night i was lying on my bed, looking
bipin at iastate.edu up at the beautiful stars, and i said to myself,
n2.bkd at isumvs.iastate.edu 'where the F*CK is my ROOF !!'