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PCR for mycoplasma infe

KUN Celbiologie kuncelb3 at caos.kun.nl
Mon Jul 25 06:24:11 EST 1994

slee at opal.tufts.edu writes:

>Hi.  Does anyone have experience with using PCR to detect mycoplasma 
>infections in tissue culture cells?  Do you havwe oligo sequences that work?
>Does anyone do this method routinely?  Is it sensitive?  Does anyone know
>the commercial vendor that sells the PCR kit for mycoplasma detection?  Thanks
>so much Steph

We recently purchased a (rather expensive) mycoplasma pcr primer set from
Statagene (cat.# 302007), for approx. USD 300. We have not tested this set
yet, but I hope this helps for you.

Henri Molhuizen
Dep. of Dermatology
University Hospital Nijmegen
The Netherlands

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