slee at opal.tufts.edu writes:
>Hi. Does anyone have experience with using PCR to detect mycoplasma
>infections in tissue culture cells? Do you havwe oligo sequences that work?
>Does anyone do this method routinely? Is it sensitive? Does anyone know
>the commercial vendor that sells the PCR kit for mycoplasma detection? Thanks
>so much Steph
We recently purchased a (rather expensive) mycoplasma pcr primer set from
Statagene (cat.# 302007), for approx. USD 300. We have not tested this set
yet, but I hope this helps for you.
Dep. of Dermatology
University Hospital Nijmegen