Clontech 5'-RACE kit
hhwoo at ccit.arizona.edu
Mon Jul 25 16:03:34 EST 1994
I have been using the Clontech 5'-amplifinder kit. It was very efficient to
get a full length cDNA. I could get a full length sequence in less than a week.
I cloned the full length cDNAs encoding ubiquitin conjugating enzyme (Gene, in
press) and H1 histone (BBA, in press) by 5'-amplifinder. It took less than a
week and was quite easy.
I do not understand the people who is having a trouble with this kit.
The most problems, I think, come from the degraded RNA or improper PCR primers.
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