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HELP with in vitro enz assays

Francisco J. Ayala ayala at biosun.harvard.edu
Mon Jul 25 13:20:20 EST 1994


hello -- i'm struggling with an in vitro kinetic assay of G3PDH.  I have
protocols and a state-of-the-art spectrophotometer, but when i try
to measure reaction velocity (d Absorbance / d Time) i get a curve
instead of the expected straight line.

if anyone has experience with this problem or with enzyme assays
in general, please drop me a line at z_ayala at harvard.edu.  I will 
gratefully acknowledge your help in my paper.

thanks!!
ze' ayala




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