hello -- i'm struggling with an in vitro kinetic assay of G3PDH. I have
protocols and a state-of-the-art spectrophotometer, but when i try
to measure reaction velocity (d Absorbance / d Time) i get a curve
instead of the expected straight line.
if anyone has experience with this problem or with enzyme assays
in general, please drop me a line at z_ayala at harvard.edu. I will
gratefully acknowledge your help in my paper.