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tips on casting sequencing gel

Chris Upton cupton at sol.uvic.ca
Tue Jul 26 16:16:06 EST 1994


In Article <3114a0$8lk at louie.udel.edu>, landel at helios (Carlisle Landel) wrote:
>In article <30p5nk$gvp at server.st.usm.edu>,
>Shiao Y. Wang <sywang at whale.st.usm.edu> wrote:
>>pzheng at VAXA.WEEG.UIOWA.EDU wrote:
>>: If you are still casting your own sequncing gels like me, here is a tip for 
>>: you. Put sigmacote on both plates[despite what you have read from sequencing

>>: protocols]and proceed as usual. I started this as a mistake, but it worked 
>>: beautifully everytime. This way you don't have to worry about air bubbles
and
>>: the gel will always stick to the bottom plate when you take them apart.
>>
>>One of my students made the same mistake. He couldn't pour the gel because
>>both plate were now hydrophobic and the acrylamide soln wouldn't enter between
>>the glass plates. So, proceed with caution.
>>
>>Shiao Wang
>>University of Southern Mississippi
>>
>
>
>Let me second Shiao Wang's cautionary note!  If you accidently coat both
>plates, you are in for *big* headaches in getting the gel solution between
>the plates!
>
>We notice that somehow, with time, the coating migrates to the other
>plate, and we have big problems  (We use Glassclad.)  How this happens
>is a big mystery.  However, we can get it off with 5M NaOH.
>
>Carlisle Landel
>A.I. duPont Institute
>
>-who cleaned gel plates with NaOH just yesterday.....
>
I always put the siliconizing agent on both plates - never had a problem
pouring & rarely had one separating the plates.

Cheers,
Chris Upton

Chris Upton 
Biochemistry & Microbiology
University of Victoria
PO Box 3055, Victoria
BC, Canada   V8W 3P6

(604)721-6507
(604)721-8855 fax



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