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PCR products in Southerns

Judy Broom judy at sanger.otago.ac.nz
Tue Jul 26 21:14:13 EST 1994

I would like to use PCR products as probes in Southerns (using 
the Boehringer Random Primed DNA Labeling Kit).  What purification 
of the PCR product is necessary?  Someone recommended the Bio Rad Pr
ep-A-Gel system to me - would a crude ethanol precipitation do in
stead?  (I tried probing with NO purification, and it sort of worked... )

I'd be interested to hear what works for other people.


Judy Broom   University of Otago    New Zealand

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