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Probe

MAHER DERBEL mp0ab01 at CUIPC.IPC.CHIBA-U.AC.JP
Tue Jul 26 21:32:09 EST 1994


Dear Netter

I have tryed screening two expression library using an antibody,but I could not
get any positif clone.I digested the protein separate the peptides on HPLC
Sequencing one of the peptide ,now I am entending to order synthetic Oligo-
nucleotides to use them as probe for screening.
analysing the proteine sequence,I found that I can make a 17 bp oligonucleotide
with 64 possible sequences
Could some body HELP me,telling me about the average "possible sequences" that 
researcher are using to make probe
64 possible sequences is reasonnable?
what about the cost?
I read in some books that I can use Optimum codon choise,if it's possible
how can I get the optimum codon choise when deducing a probe sequense from 
RAT Amino Acid sequence Data? is it really practical?

^^Maher
Email:mp0ab01 at cuipc.ipc.chiba-u.ac.jp



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