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mRNA quantitation

Terry Hanzlik terryh at ento.csiro.au
Tue Jul 26 19:08:51 EST 1994


Dear Tracey,
Look at Li and Brow in Nuc Acids Res 21, 4645-46 (1993).  I've
never used this technique but it looks extremely simple and
robust.  Basically all you do is hybridize a labelled oligo to total RNA
in 4 ul of buffer for half an hour then run the total on a
nondenaturing gel until the unhyb'd stuff runs off the bottom of
the gel.  Dry the gel and autoradiograph.  Do standards with T7
transcripts.  The authors' data showed linearity and sensitivity
to at least 1 fmol target.

I hope this helps.

Terry Hanzlik, terryh at ento.csiro.au
CSIRO Division of Entomology
Box 1700
Canberra, ACT 2601
Australia




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