Fixing sequencing gels

[Hong Dang]

In article <Tmeyn-260794184028 at blast.agmarley.arizona.edu>, Tmeyn at ccit.arizona.edu (Tony Meyn) writes:
|> In article <cupton.1125646336B at ra.uvic.ca>, cupton at sol.uvic.ca ( Chris
|> Upton) wrote:
|> 
|> > In response to an article in Biotechniques (FEB '94, Yang & Waldman), that
|> > suggested it wasn't necessary to fix 35S seq gels but that the Saran Wrap
|> > should be left on - I persuaded a grad student next door to try this :-)
|> > 
|> > He found it worked fine!! (to think of all the time I spent fixing gels!!)
|> > 
|> > BUT, he also took off the Saran Wrap!!  & there was _NO_ problem with the
|> > gel sticking to the film!!
|> > 
|> > This may be because he dried the gels very well or low local humidity.
|> > I guess it's something to test yourself.
|> > 
|> > All in all he was pretty happy with it.
|> > 
|> > Cheers,
|> >   Chris
|> > 
|> 
|> Chris-
|> 
|> I never fix my sequencing gels.  You can not use Saran Wrap between
|> the film and the gel as S35 is too weak to penetrate the plastic.  
|> I dry my gels at 80C for two hours.  Normally this is fine.  
|> When the humidity get up here, though, (around 40% :) )the film
|> does tend to stick somewhat.  This doesn't bother me too much as
|> I can still read the sequence without a problem.  
|> 
|> Tony Meyn
|> University of Arizona

Use some baby powder before putting down film. Save you 1 hr drying time.

Hong