Electro-blotting of proteins

[Dietmar.Tietz at agrar.uni-giessen.de]

> 
> 
> Hi everyone,
> 
> I would like to ask whether anyone out there has a good protocol for the 
> electro-blot transfer of proteins. I would specifically like to know the 
> composition of the blotting buffer, type of membrane and running parameters. 
> The proteins that I would like to blot are in vitro synthesized biotinylated 
> peptides of up to 100 kDa. 
> 
> Rob van der Luijt
> MGC-Dept. of Human Genetics
> Leiden University
> Leiden, The Netherlands
> Tel.:  31-71-276097
> Fax.: 31-71-276075
> E-mail: rob at ruly46.leidenuniv.nl
> 
> 
> 
You may want to study this review:

J. M. Gershoni: Protein blotting: a tool for the analytical biochemistry.
Advances in Electrophoresis (VCH), 1, 141-175 (1987)

Good luck!


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*           Dietmar Tietz, Ph.D.,  Research Scientist            *
*            Biostatistics, Justus-Liebig-University             *
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