I would like to ask whether anyone out there has a good protocol for the
electro-blot transfer of proteins. I would specifically like to know the
composition of the blotting buffer, type of membrane and running parameters.
The proteins that I would like to blot are in vitro synthesized biotinylated
peptides of up to 100 kDa.
Rob van der Luijt
MGC-Dept. of Human Genetics
Leiden, The Netherlands
E-mail: rob at ruly46.leidenuniv.nl