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Electro-blotting of proteins

Riccardo Fodde fodde at ruly46
Wed Jul 27 12:42:39 EST 1994


Hi everyone,

I would like to ask whether anyone out there has a good protocol for the 
electro-blot transfer of proteins. I would specifically like to know the 
composition of the blotting buffer, type of membrane and running parameters. 
The proteins that I would like to blot are in vitro synthesized biotinylated 
peptides of up to 100 kDa. 

Rob van der Luijt
MGC-Dept. of Human Genetics
Leiden University
Leiden, The Netherlands
Tel.:  31-71-276097
Fax.: 31-71-276075
E-mail: rob at ruly46.leidenuniv.nl



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