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RNA gels

LMS lee.smith at afrc.ac.uk
Wed Jul 27 05:20:28 EST 1994


Help
I'm about do do my first RNA gel/Northern blot and I'm confused.
Why does everybody use formamide gels instead of glyoxal/DMSO
(which according to Maniatis give sharper bands)?
I would prefer not to use formamide as we don't have a fume
hood in the lab.
Any suggestions?
Any other ideas always welcome!
LEE SMITH, Institute for Animal Health,Compton




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