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Fixing sequencing gels

Manuel G. CLAROS claros at biologie.ens.fr
Thu Jul 28 05:34:43 EST 1994


In Article <Tmeyn-260794184028 at blast.agmarley.arizona.edu>,
Tmeyn at ccit.arizona.edu (Tony Meyn) wrote:

>I never fix my sequencing gels.  You can not use Saran Wrap between
>the film and the gel as S35 is too weak to penetrate the plastic.  
>I dry my gels at 80C for two hours.  Normally this is fine.  

Using a regular pump, 45  min should be enougth!!!!!

And I never fixed my 35S sequencing gels since 1989 ;-)

I hope this "improves"

Manuel G.

M. G. Claros ------------------------------- claros at biologie.ens.fr
Ecole Normale Superieure  Genetique Moleculaire                      |/    \|
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