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5' cDNA question

slee at opal.tufts.edu slee at opal.tufts.edu
Thu Jul 28 09:09:03 EST 1994


Dear netters:  does anyone one know a sure fire method of determining the 
5' end of a cDNA.  How do you know whether the site you map by primer ex-
tension, s1 nuclease is the TSS or an intron?  This is especially hard
to figure out in TATA-less receptor cDNA's  I would appreciate any
ideans and opinions.  Steph e-mail "slee at opal.tufts.edu"  Thanks.


p[2O0le



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