NA45 Paper DNA extraction problem

Carlisle Landel landel at helios
Fri Jul 29 11:49:10 EST 1994


>grieger at matt.ksu.ksu.edu (David M Grieger) writes:
>
>>I had my student try to purify a 200bp fragment from a gel using the NA45
>>paper technique, recently discussed here on this news group.  After she did
>>the hi-salt washes and the EtOH precipitation (+glycogen), there was no
>>normal pellet but a nonaqueous "bubble" in the bottom of the microfuge tube.
>> After adding TE and doing a second EtOH precipitation the same result
>>occured.  Could this result from carry over from the hi-salt washes?  Any
>>thoughts on this problem would be appreciated.  Thanks.
>

Do you have any phospate buffer around?  It sounds like the bubble you
get when you try to EtOH ppt from phospate buffer.

Carlisle Landel





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