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NA45 Paper DNA extraction problem

Carlisle Landel landel at helios
Fri Jul 29 11:49:10 EST 1994

>grieger at matt.ksu.ksu.edu (David M Grieger) writes:
>>I had my student try to purify a 200bp fragment from a gel using the NA45
>>paper technique, recently discussed here on this news group.  After she did
>>the hi-salt washes and the EtOH precipitation (+glycogen), there was no
>>normal pellet but a nonaqueous "bubble" in the bottom of the microfuge tube.
>> After adding TE and doing a second EtOH precipitation the same result
>>occured.  Could this result from carry over from the hi-salt washes?  Any
>>thoughts on this problem would be appreciated.  Thanks.

Do you have any phospate buffer around?  It sounds like the bubble you
get when you try to EtOH ppt from phospate buffer.

Carlisle Landel

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