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dNTP concentration in PCR

Dick Vogt vogt at tbone.biol.scarolina.edu
Fri Jul 29 19:18:37 EST 1994

>[dNTPs] in PCR?  My neighbor does differential display (in which, for
>the first round they only need to detect the product on an autorad) at
>2uM each.  I guess you don't get EtBr-stainable bands with this
>concentration, but he says you do with 20uM. 

Gee, is this going to be sociological?  I use 50 pmoles each primer scaled 
to a 100 ul PCR reaction.
Richard Vogt (Internet: vogt at biol.scarolina.edu) (803) 777-8998
Department of Biology, University of South Carolina, Columbia SC 29208

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