with the : > gel sticking to the film!!
: > This may be because he dried the gels very well or low local humidity.
: > I guess it's something to test yourself.
: > All in all he was pretty happy with it.
: > Cheers,
: > Chris
X-Newsreader: TIN [version 1.2 PL2]
: I never fix my sequencing gels. You can not use Saran Wrap between
: the film and the gel as S35 is too weak to penetrate the plastic.
: I dry my gels at 80C for two hours. Normally this is fine.
: When the humidity get up here, though, (around 40% :) )the film
: does tend to stick somewhat. This doesn't bother me too much as
: I can still read the sequence without a problem.
: Tony Meyn
: University of Arizona
I have used this technique successfully "way down south" where humidity
is oppressive, about 85% right now. I've found that "Cling Wrap" works
immensly (sp?) better than "Saran Wrap" - don't know why. I put the gel
on filter paper, cover with Cling, and dry 2 hours at 80C. The Cling
pulls off with no problems - never had a problem with sticking. Peace...
johnsbs at mail.auburn.edu