In Article <Tmeyn-260794184028 at blast.agmarley.arizona.edu>,
Tmeyn at ccit.arizona.edu (Tony Meyn) wrote:
>In article <cupton.1125646336B at ra.uvic.ca>, cupton at sol.uvic.ca ( Chris
>>> In response to an article in Biotechniques (FEB '94, Yang & Waldman), that
>> suggested it wasn't necessary to fix 35S seq gels but that the Saran Wrap
>> should be left on - I persuaded a grad student next door to try this :-)
>>I never fix my sequencing gels. You can not use Saran Wrap between
>the film and the gel as S35 is too weak to penetrate the plastic.
>I dry my gels at 80C for two hours. Normally this is fine.
>When the humidity get up here, though, (around 40% :) )the film
>does tend to stick somewhat. This doesn't bother me too much as
>I can still read the sequence without a problem.
>University of Arizona
I don't fix sequencing my gels anymore either-don't need to. However, I find
that I can leave the Saran Wrap on 35S sequencing gels and get good signal
from an overnight exposure nearly every time. I have also found that one
hour of drying at 80C is usually long enough, unless yo run wedge gels.