IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Fixing sequencing gels

Tracy Aquilla aquilla at salus.med.uvm.edu
Sat Jul 30 15:50:10 EST 1994


In Article <Tmeyn-260794184028 at blast.agmarley.arizona.edu>,
Tmeyn at ccit.arizona.edu (Tony Meyn) wrote:
>In article <cupton.1125646336B at ra.uvic.ca>, cupton at sol.uvic.ca ( Chris
>Upton) wrote:
>
>> In response to an article in Biotechniques (FEB '94, Yang & Waldman), that
>> suggested it wasn't necessary to fix 35S seq gels but that the Saran Wrap
>> should be left on - I persuaded a grad student next door to try this :-)
...(stuff deleted)... 
>> Cheers,
>>   Chris
>> 
>
>Chris-
>
>I never fix my sequencing gels.  You can not use Saran Wrap between
>the film and the gel as S35 is too weak to penetrate the plastic.  
>I dry my gels at 80C for two hours.  Normally this is fine.  
>When the humidity get up here, though, (around 40% :) )the film
>does tend to stick somewhat.  This doesn't bother me too much as
>I can still read the sequence without a problem.  
>
>Tony Meyn
>University of Arizona

I don't fix sequencing my gels anymore either-don't need to. However, I find
that I can leave the Saran Wrap on 35S sequencing gels and get good signal
from an overnight exposure nearly every time. I have also found that one
hour of drying at 80C is usually long enough, unless yo run wedge gels.
    Tracy



More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net