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dNTP concentration in PCR (fwd)

Robert Preston rapr at MED.PITT.EDU
Sun Jul 31 01:34:02 EST 1994


> 
> >[dNTPs] in PCR?  My neighbor does differential display (in which, for
> >the first round they only need to detect the product on an autorad) at
> >2uM each.  I guess you don't get EtBr-stainable bands with this
> >concentration, but he says you do with 20uM. 
> 
> Gee, is this going to be sociological?  I use 50 pmoles each primer scaled 
> to a 100 ul PCR reaction.
> -- 
> Richard Vogt (Internet: vogt at biol.scarolina.edu) (803) 777-8998
> Department of Biology, University of South Carolina, Columbia SC 29208
> 
Well, so what?  Did you think primers are the same as dNTP, or something? ;-)
Rob Preston
rapr at med.pitt.edu




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