>> >[dNTPs] in PCR? My neighbor does differential display (in which, for
> >the first round they only need to detect the product on an autorad) at
> >2uM each. I guess you don't get EtBr-stainable bands with this
> >concentration, but he says you do with 20uM.
>> Gee, is this going to be sociological? I use 50 pmoles each primer scaled
> to a 100 ul PCR reaction.
> --
> Richard Vogt (Internet: vogt at biol.scarolina.edu) (803) 777-8998
> Department of Biology, University of South Carolina, Columbia SC 29208
>Well, so what? Did you think primers are the same as dNTP, or something? ;-)
Rob Preston
rapr at med.pitt.edu