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Rain-ex on sequencing plates?

neale at mbcf.stjude.org neale at mbcf.stjude.org
Fri Jul 29 19:14:03 EST 1994


In article <313vrn$j2g at nic.umass.edu>, JMANGOR at UCSVAX.UCS.UMASS.EDU (Jodie Mangor) writes:
> I would appreciate any advice on the use of Rain-ex on sequencing plates.
> Thank you!
> 
> Jodie Mangor
> UMASS


We routinely use Rain-ex on our sequencing plates, and it works great. We use
0.4 mm spacers, and apply Rain-ex to ONE plate only. A grad student applied it
to both plates and could not get the gel solution to flow between the
plates--so beware!

We usually apply to the larger plate so that the gel will stick to it. Works
like a charm, no toxicity to worry about. Just follow the directions on the
bottle--apply two coats, allowing it to dry between applications.

Wash plates after electrophoresis as usual. Lasts for approx 15 to 20 gels,
then reapply to clean plates.

Cheers,


Geoff Neale

Dept. of Virology and Molecular Biology        Internet: neale at mbcf.stjude.org
St. Jude Children's Research Hospital             Phone: (901) 522-0400
Memphis, TN                                         Fax: (901) 523-2622





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