In article <313vrn$j2g at nic.umass.edu>, JMANGOR at UCSVAX.UCS.UMASS.EDU (Jodie Mangor) writes:
> I would appreciate any advice on the use of Rain-ex on sequencing plates.
> Thank you!
>> Jodie Mangor
> UMASS
We routinely use Rain-ex on our sequencing plates, and it works great. We use
0.4 mm spacers, and apply Rain-ex to ONE plate only. A grad student applied it
to both plates and could not get the gel solution to flow between the
plates--so beware!
We usually apply to the larger plate so that the gel will stick to it. Works
like a charm, no toxicity to worry about. Just follow the directions on the
bottle--apply two coats, allowing it to dry between applications.
Wash plates after electrophoresis as usual. Lasts for approx 15 to 20 gels,
then reapply to clean plates.
Cheers,
Geoff Neale
Dept. of Virology and Molecular Biology Internet: neale at mbcf.stjude.org
St. Jude Children's Research Hospital Phone: (901) 522-0400
Memphis, TN Fax: (901) 523-2622
