Transgenic constructs - HELP!!!

Dominic Voon dvoon at uniwa.uwa.edu.au
Thu Jun 2 01:16:09 EST 1994


We want to inject linearised plasmid to produce transgenic mice, rather
than remove the vector sequences.  Is this OK??  We have double
CsCl-purified the DNA, but  how do we  clean the
DNA after REnz digestion - ethanol precipitation??

PLEASE HELP - many thanks.  If you know someone who may help, please
refer them to us. 

Please reply by EMail. 

 REGARDS. 




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