RNase inhibitor

Shahram Mori smori at opus.nmsu.edu
Thu Jun 2 00:17:57 EST 1994


wzhao at phy.bcm.tmc.edu wrote:
: Hi, netters:

: I have been doing in vitro transcription by T7 polymerase recently.
: I am wondering if the RNase inhibitor is critical or essential 
: for the transcription. Any advice is greatly appreciated.

: Wei
Its Extremely important. rRNasin is used at a concentration of 20u, if I
recall correctly. 
Cheers.
Shahram Mori
Program in Molecular Biology
Dept. of Chemistry and Biochemistry
NMSU Las Cruces NM
88003

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