Cutting plasmid inside inverted repeats?

Shahram Mori smori at opus.nmsu.edu
Thu Jun 2 00:03:30 EST 1994


Ed Beaty (ed_beaty at qms1.life.uiuc.edu) wrote:
: Hello again, again...

: I think I figured out why I can't cut the inverted repeats in my plasmid
: with restriction endonucleases:  The reference is D.M. Lilley and L.R.
: Hallam, J Biomol Struct Dyn 1: 169-82 (1983).  The abstract says that some
: inverted repeats in negatively supercoiled plasmids may form a cruciform
: structure that is resistant to cleavage by restriction enzymes, but is
: hypersensitive to single-strand-specific nucleases like S1.  

That's excatly what I was gonna say!!!!!!!! :)
I am glad you found the answer.
Cheers.
Shahram Mori
Program in Molecular Biology
Dept. of Chemistry and Biochemistry
Box 3C NMSU
Las Cruces NM
88003


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