electroporation cuvette

Manuel Simon ma at ABC.univie.ac.at
Wed Jun 1 11:29:34 EST 1994


In article <mdhouse-180594162503 at bark-632.berkeley.edu> mdhouse at mendel.berkeley.edu (Schekman Lab) writes:
>From: mdhouse at mendel.berkeley.edu (Schekman Lab)
>Subject: Re: electroporation cuvette
>Date: Wed, 18 May 1994 16:25:03 -0800

>In article <01HCHOU7R2DU8WWBLA at SKYCAT.USask.CA>, LEES at SASK.USASK.CA wrote:

>> I remember there were a lot of discussions on recycling of 
>> electroporation cuvettes. What was the best way to recycle used 
>> cuvettes?

>Best is to thoroughly wash with distilled water and keep them under 70%
>EtOH shortly before use. Dry them invertedly on some sterile filterpaper
>for a few minutes. If sterility is an absolute must, use sterile filtered
>EtOH because some fungal spores can remain viable in 70% EtOH. In practise
>I never got any fungal contaminants though. You can recycle
>e-porationcuvettes a few times. If they have been used often, electrodes
>corrode and give poor transformation efficiency. 

This is a particular problem since sometimes one does not see the loss of 
proper contact due to corrosion and gets therefore very inefficient 
tranfections without any (obvious) reason whereas new cuvettes give good 
results!


>Good luck,




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