Screen a library for amidase?

hetherington at mbcf.stjude.org hetherington at mbcf.stjude.org
Wed Jun 1 12:04:44 EST 1994


Looking for amidase screen.

I want to build a bacterial expression library and screen colonies for amidase
activity.  I have tried an agar overlay method modified from the standard
urease test, but with no results in a positive control.  

Anyone have experience with a colony amidase screening method that works?




More information about the Methods mailing list