Screen a library for amidase?

hetherington at hetherington at
Wed Jun 1 12:04:44 EST 1994

Looking for amidase screen.

I want to build a bacterial expression library and screen colonies for amidase
activity.  I have tried an agar overlay method modified from the standard
urease test, but with no results in a positive control.  

Anyone have experience with a colony amidase screening method that works?

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