Slanted Bands on Agarose Gels

Mike Morris mike at divsun.unige.ch
Wed Jun 1 09:47:23 EST 1994


In article 206 at usenet.ucs.indiana.edu, wfischer at bio.indiana.edu (Will Fischer) writes:
*Christopher W Botka (cwbotka at MERCURY.SFSU.EDU) wrote:
*: Has anyone experienced bands(DNA) that run slanted on an agarose gel? -
*: i.e. the bottom of the EtBr stained frag. of DNA seems to be migrating
*: faster than the top. 
*
*  Seems the culprit is different buffer conc. between gel and
*running buffer, generally due to volume loss while dissolving the agarose.
*
*
	I have always believed that it was due to a TEMPERATURE gradient in the gel, not buffer concentration. I avoid it by running gels more slowly, or 
in the cold room.

*******************************************************************
Michael Morris PhD            
Division of Medical Genetics       tel (Switzerland) (22) 702.56.94
CMU, University of Geneva          fax (Switzerland) (22) 702.57.06
Geneva, Switzerland                email mike at medsun.unige.ch                  




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