PCR of episomal plasmids

Rosemary Lemons rmlemons at um
Wed Jun 1 07:25:14 EST 1994


Any hints on how to PCR plasmids that have been transfected into
fibroblasts?
We know they're in there but have yet to obtain any PCR products.  I can
get
product using just a plasmid at the femptagram level so I believe I've
optimized conditions, but still nothing from the cells.  Any thoughts would
be
greatly appreciated as we're a bit new to this area.



More information about the Methods mailing list