Hints for PCR of A/T-rich sequences?

NA medp3054 at leonis.nus.sg
Thu Jun 2 02:24:08 EST 1994


: >I have to do a bunch of PCR on highly A/T rich sequences and was wondering
: >if anyone has done any optimization experiments for any of the zillion PCR
: >variables, as they relate to A/T sequences.  Lower extension temp? Higher salt?
: >Increase gelatin?  Any thoughts on this would be appreciated.

Hi there... you might want to try TMAC (tetramethyl ammonium chloride)... 
at 3 M, the A-T binding is of similar strength of that of G-C. TMAC 
enhances A-T binding over that of G-C binding by stabilizing the A-T 
bonds...  





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