RNase inhibitor

Hinayana Bawagan bawagan at umdnj.edu
Thu Jun 2 14:48:28 EST 1994

smori at opus.nmsu.edu (Shahram Mori) writes:

>wzhao at phy.bcm.tmc.edu wrote:
>: Hi, netters:

>: I have been doing in vitro transcription by T7 polymerase recently.
>: I am wondering if the RNase inhibitor is critical or essential 
>: for the transcription. Any advice is greatly appreciated.

>: Wei
>Its Extremely important. rRNasin is used at a concentration of 20u, if I
>recall correctly. 
>Shahram Mori
>Program in Molecular Biology
>Dept. of Chemistry and Biochemistry
>NMSU Las Cruces NM

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     I used RNAsin at 40U/ul conc. 

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