High MWt Markers
udbl119 at bay.cc.kcl.ac.uk
Thu Jun 2 18:37:22 EST 1994
I am working on a protein with a molecular weight of 320kD and routinely do
Westerns to probe for this protein. My concern however is that I use both
Bio-rad and Sigma Broad range markers of which the largest protein is rabbit
skeletal muscle myosin which has a mwt of 200kD, and so when it comes to
determining the mwt I have to extrapolate back to determine the molecular
weight which isn't terribly accurate!. I am therefore looking for a protein
with a mwt greater than 300kD that I can add to my markers. Does anyone know
of a commercially available protein that has a mwt greater than 300kD I could
add to my markers. Alternatively do you know of prepared mwt markers that I
could buy that have greater range. Cheers.
(M.Johnstone at bay.cc.kcl.ac.uk)
King's College, London.
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