Total Digest of Proteins

[Henrik Kibak]

I thought perhaps you molbio types who usually want to get rid of
protein might be able to offer some suggestions...

In order to determine if the molecule I am interested in is a peptide,
I would like to determine if protease digestion will abolish activity.

I have tried digesting my crude (animal tissue) homogenate with 1 mg/ml
pronase (24 hr, room temperature, up to 200 ml total volume), but this
enzyme alone does not completely digest all proteins to amino acids. 

Can anyone suggest a protease or combination of proteases which will
do the job?

Thanks,
-Beth Schomer

POSTED BY hkibak at leland.stanford.edu FOR schomer at leland.stanford.edu
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