polyacrylamide-agarose gel

[futers at bpxtal.leeds.ac.uk]

In article <9406012128.AA16466 at acs2.acs.ucalgary.ca>, 
llintott at ACS.UCALGARY.CA ("Lauri Lintott") writes:
>Has anyone out there ever used vertical acrylamide-agarose gels?
>I am trying to make a 3.5% acrylamide/0.5% agarose gel but I am
>having 2 problems.  First of all how do you keep the gel from
>slideing out?  I have tried using a 2% agarose wedge at the
>bottom but this was unsuccesful.  Secondly, how do you get the
>comb out without the wells collapsing?
>One more question, do the spacers have to be thicker than the
>comb as is stated in the only procedure I can find (R. H. Nicolas
>in Gel Electrophoresis of Nucleic Acids:A Practical Approach.
>1990.  Ed. D. Rickwood and B.D. Hames).
>Any advice would be appreciated.
>
>Lauri 
>

Hi Lauri,
I have used a 4% acrylamide/0.7% agarose gel without the problems you 
describe.  The slightly higher percentage might help.
           Simon Futers (futers at biovax.leeds.ac.uk)