Kanamycin inhibits EcoR1?!

???? maria maria at divsun.unige.ch
Thu Jun 2 11:18:11 EST 1994


Very strange problem: we were unable to cut some cosmids with
EcoR1 (but enzyme worked fine on old cosmids, and other enzymes
worked on these cosmids). We traced this to (I know it sounds
unbelievable, but its TRUE!) the kanamycin used for the culture:
an old, many many times-frozen/thawed tube was apparently "EcoR1-
toxic", with a new tube no more problem.
  Does anybody have a (even remotely) rational explanation for this,
or am I just getting old?
 tia, Maria



More information about the Methods mailing list