DNA PAGE EtBr query.

[Michael van Waes]

>
>Netfolks: do any of you know if EtBr can be successfully included
>in a polyacrylamide gel, and/or in the running buffer, to avoid
>the post-run immersion in staining solution?  (and does enough
>excitation light get through the glass plate from a UV-box?)
>
>Thanks for input (neither current protocols nor maniatis specifically
>rule out running PAGE with EtBr).
>
>Rob Preston
>rapr at med.pitt.edu
>
The answer to that is yes, just like with agarose you can add it before
the TEMED/AP.
	Cheers.				Michael van Waes.