DDRT-PCR/tiny samples

[Ward Lab]

We are trying to find out how little RNA can be used for successful differential
display RT-PCR.  Specifically, we would like to use RNA from one or a very small
number (100) of cells (spermatocytes from C. elegans).  Has anyone used such
limited samples for this type of experiment, or does anyone have a good protocol
for isolating RNA from such small samples.  Please reply here or to:
Paul Muhlrad or Craig LaMunyon
University of Arizona
Department of Molecular and Cellular Biology
Tucson, AZ  85721
phone (602) 621-9135
fax (602)621-3709
email Ward_lab at tikal.biosci.arizona.edu

Thanks