HELP: Screening bact.-colonies with Ab?

Troels Wind wind at biobase.aau.dk
Thu Jun 2 08:07:15 EST 1994


Hi there,
	Does anybody out there know if it is possible to make a colony-
lift from agar-plates, lyse the bacteria and detect proteins integrated
in the inner membrane with Ab? The antigenic part of the protein is in 
the periplasmic space.
	If so, please send me a brief protocol.
		
				Thanks in advance
				wind at biobase.aau.dk




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