Water used in PCR

4700gbera at UMBSKY.CC.UMB.EDU 4700gbera at UMBSKY.CC.UMB.EDU
Fri Jun 3 14:01:21 EST 1994


Hello Netters-

I'm having real trouble consistantly amplifying an 1880 bp fragment
from bacteria. My first couple of trys worked beautifully. Then the 
product pretty much disappeared. (except for one run a few days ago,
this is really driving me nuts)  I've gone through the usual suspects -
Taq, primer, 10x, Mgcl, etc etc. Right now I'm running a temperature 
recorder on our thermocycler. I'm also wondering about our water supply. 

My question - what kind of water do you folks out there use for PCR??
Has anyone run into any problems with it?


Thanks - 

Gina Berardesco
4700gbera at umbsky.cc.umb.edu
University of Massachusetts, Boston








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