High MWt Markers
Chanpen Wiwat - PYMI
pycww at MUCC.MAHIDOL.AC.TH
Fri Jun 3 06:21:46 EST 1994
On Thu, 2 Jun 1994, Mandy Johnstone wrote:
> Hi Folks,
> I am working on a protein with a molecular weight of 320kD and routinely do
> Westerns to probe for this protein. My concern however is that I use both
> Bio-rad and Sigma Broad range markers of which the largest protein is rabbit
> skeletal muscle myosin which has a mwt of 200kD, and so when it comes to
> determining the mwt I have to extrapolate back to determine the molecular
> weight which isn't terribly accurate!. I am therefore looking for a protein
> with a mwt greater than 300kD that I can add to my markers. Does anyone know
> of a commercially available protein that has a mwt greater than 300kD I could
> add to my markers. Alternatively do you know of prepared mwt markers that I
> could buy that have greater range. Cheers.
> Mandy Johnstone
> (M.Johnstone at bay.cc.kcl.ac.uk)
> King's College, London.
I would like to suggest you a high molecular weigth kit from Pharmacia.
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