High MWt Markers

[Chanpen Wiwat - PYMI]

On Thu, 2 Jun 1994, Mandy Johnstone wrote:

> Hi Folks,
> I am working on a protein with a molecular weight of 320kD and routinely do 
> Westerns to probe for this protein.  My concern however is that I use both 
> Bio-rad and Sigma Broad range markers of which the largest protein is rabbit 
> skeletal muscle myosin which has a mwt of 200kD, and so when it comes to 
> determining the mwt I have to extrapolate back to determine the molecular 
> weight which isn't terribly accurate!. I am therefore looking for a protein 
> with a mwt greater than 300kD that I can add to my markers.  Does anyone know 
> of a commercially available protein that has a mwt greater than 300kD I could 
> add to my markers.  Alternatively do you know of prepared mwt markers that I 
> could buy that have greater range.  Cheers.
> 
> Mandy.
> 
> 
> 
> _______________
> Mandy Johnstone
> (M.Johnstone at bay.cc.kcl.ac.uk)
> 
> King's College, London.
> 
Dear Mandy,

I would like to suggest you a high molecular weigth kit from Pharmacia. 

Good luck.
Chanpen>