Protocols for isolating large (>200kb) plasmids.

[goyal at mbcl.rutgers.edu]

Dear netters,
	I am looking for protocols for isolating 'mega' plasmids (200kb or more)
from bacteria. I have tried some of the usual alkaline lysis procedures, but
havent yet succeeded fully.
	Before I test a couple of other procedures, I thought I would get input
from others who might have had some experience in this.

Thanks

ANIL GOYAL

Goyal at mbcl.rutgers.edu