Protocols for isolating large (>200kb) plasmids.

goyal at mbcl.rutgers.edu goyal at mbcl.rutgers.edu
Thu Jun 2 15:39:16 EST 1994


Dear netters,
	I am looking for protocols for isolating 'mega' plasmids (200kb or more)
from bacteria. I have tried some of the usual alkaline lysis procedures, but
havent yet succeeded fully.
	Before I test a couple of other procedures, I thought I would get input
from others who might have had some experience in this.

Thanks

ANIL GOYAL

Goyal at mbcl.rutgers.edu



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