Quick and cheap +/- PCR screen?

bagley at fauna.ucdavis.edu bagley at fauna.ucdavis.edu
Fri Jun 3 22:10:38 EST 1994


Can anyone recommend a quick and cheap procedure to identify whether a PCR
reaction worked or not?  The size of the product, when it amplifies, is known
so running it out on a gel seems wasteful.  I suspect diagnostic laboratories
must have a simple procedure to do this.  Mixing the PCR  reaction together 
with dilute ethidium bromide and dotting this over UV light does not seem 
to work well, presumably because unincorporated primers flouresce strongly.
Any ideas that do not include lots of tips, tubes, or $20,000 equipment will
be appreciated!
Mark.



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