"universal" positive control

Walter Hill walterh at U.WASHINGTON.EDU
Fri Jun 3 18:04:36 EST 1994


I would appreciate it if someone could suggest a good positive control 
PCR target that would be present in all genomic DNA preps from marine 
vertebrates and/or marine invertebrates.  I seen to recall something 
about a conserved region of an rRNA gene that might be appropriate but, 
unfortunately, cant' remember any details.  The purpose of this target 
would be to assure that amplifiable nucleic acids have been extracted and 
that the reaction is sufficient free of inhibitors to get sufficient 
amplification to produce a visible products.
TIA for any comments, suggestions and/or recommendations.
Regards, -Walt Hill

walterh at u.washington.edu (primary)   (secondary) hillw at par66.par.ora.fda.gov
Food & Drug Admin., Seafood Products Research Center, Bothell, WA 98041-3012
Affiliate Associate Professor of Microbiology       University of Washington
VOICE:(206) 483-4892           "Cook it first"            (206) 483-4996:FAX 




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