Transfection of Sf9/Sf21 Cells

Garry Myers garry at menzies.su.edu.au
Sat Jun 4 03:10:26 EST 1994


Hi,

I've recently become the proud owner of the CLONTECH BacPAK expression
system ;).  The protocol accompanying the kit suggests using Lipofectin(tm)
to transfect the Sf21 cells with recombinant virus.  I'm interested to hear 
any thoughts or opinions from baculoviral-minded individuals on other 
methods of transfecting Sf9/Sf21 cells, particularly electroporation.

Cheers,

Garry.

___________________________________________________________________
Garry Myers                       Menzies School of Health Research
Molecular Genetics Unit           garry at menzies.su.edu.au
___________________________________________________________________



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