Transfection of Sf9/Sf21 Cells

[Hinayana Bawagan]

garry at menzies.su.edu.au (Garry Myers) writes:

>Hi,

>I've recently become the proud owner of the CLONTECH BacPAK expression
>system ;).  The protocol accompanying the kit suggests using Lipofectin(tm)
>to transfect the Sf21 cells with recombinant virus.  I'm interested to hear 
>any thoughts or opinions from baculoviral-minded individuals on other 
>methods of transfecting Sf9/Sf21 cells, particularly electroporation.

>Cheers,

>Garry.

>___________________________________________________________________
>Garry Myers                       Menzies School of Health Research
>Molecular Genetics Unit           garry at menzies.su.edu.au
>___________________________________________________________________

     This article may be useful to you.

Journal of General Virology (1989, 70, 3501-3505.

Efficient transfection of insect cells with baculovirus DNA using
electroporation. By Susan G. Mann and Linda A. King.