Transfection of Sf9/Sf21 Cells
bawagan at umdnj.edu
Mon Jun 6 19:26:38 EST 1994
garry at menzies.su.edu.au (Garry Myers) writes:
>I've recently become the proud owner of the CLONTECH BacPAK expression
>system ;). The protocol accompanying the kit suggests using Lipofectin(tm)
>to transfect the Sf21 cells with recombinant virus. I'm interested to hear
>any thoughts or opinions from baculoviral-minded individuals on other
>methods of transfecting Sf9/Sf21 cells, particularly electroporation.
>Garry Myers Menzies School of Health Research
>Molecular Genetics Unit garry at menzies.su.edu.au
This article may be useful to you.
Journal of General Virology (1989, 70, 3501-3505.
Efficient transfection of insect cells with baculovirus DNA using
electroporation. By Susan G. Mann and Linda A. King.
More information about the Methods