Transfection of Sf9/Sf21 Cells

polk at molbiol.ox.ac.uk polk at molbiol.ox.ac.uk
Mon Jun 6 04:37:06 EST 1994


In article <garry.23.0011B4AB at menzies.su.edu.au>, garry at menzies.su.edu.au (Garry Myers) writes:
> Hi,
> 
> I've recently become the proud owner of the CLONTECH BacPAK expression
> system ;).  The protocol accompanying the kit suggests using Lipofectin(tm)
> to transfect the Sf21 cells with recombinant virus.  I'm interested to hear 
> any thoughts or opinions from baculoviral-minded individuals on other 
> methods of transfecting Sf9/Sf21 cells, particularly electroporation.
> 
> Cheers,
> 
> Garry.
> 
> ___________________________________________________________________
> Garry Myers                       Menzies School of Health Research
> Molecular Genetics Unit           garry at menzies.su.edu.au
> ___________________________________________________________________



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