Cross-linking

mazella mazella at naxos.unice.fr
Mon Jun 6 05:09:46 EST 1994


>Hia,
>I wish to cross-link a virus capsid as it assembles, and need a=
 method of=20
>chemically crosslinking almost instanoeusly...... any advances on=
 the=20
>1981'LDH with gluteraldehyde' method study by Hermann, et al, in=
 Biochemistry=20
>(20) pp1595-5201...
>
>your reply gratefully anticipated
>
>Dave Scott.
>
>gends%south-01.novell.leeds.ac.uk at gps.leeds.ac.uk
>or
>scott at uk.ac.leeds.bio.vax

"Hia",
you can also use N-hydroxysuccinimide ester and derivatives. You=
 can get a
good review in Biochimica et Biophysica acta 559 (1979) 39-69. Pierce=
 have
also made a very good technical book.

good luck.

Georges=20

----------------------------------------------------------------------------=
----
Georges GAUDRIAULT
Institut de pharmacologie mol=E9culaire et cellulaire
660, route des lucioles
06560 valbonne
=46RANCE
Tel n=B0:19 (33)-93-95-77-61
Tel n=B0:19 (33)-93-95-77-08
e-mail :mazella at naxos.unice.fr
----------------------------------------------------------------------------
----





More information about the Methods mailing list