Purification of small DNA

Richard Dana rcdana at crash.cts.com
Tue Jun 7 01:25:34 EST 1994

The purification a small DNAs from gels and solutions is quite
simple using GLASSFOG, a silica matrix, and a chaotropic salt but
sometimes it is difficult to remove the glassfines from the purified
product. The use of SpinFilters (which are available from a numver of 
vendors, including BIO 101) gets rid of these fines, and makes working
with 10-200 b.p. sized single-stranded and double-stranded DNA even
easier. The yields are good and there are a number of companies with
kits on the market but the one from BIO 101 specifically addresses the
small sized DNA fragments.
Richard Dana
BIO 101

More information about the Methods mailing list