Weird kinased oligo pattern...
G K L Munro
bssgklm at midge.bath.ac.uk
Tue Jun 7 04:57:47 EST 1994
I have been having some trouble with oligos which I am using for PCR and
sequencing. Finally last week I kinased them up to have a look (Iknow, I
should have done it straight away!). Anyway, although there is a reasonably
sized band at the right size for the oligo, there are ladders extending in
each direction!!! The oligos are 19mer, 22mer and a 25mer. They were
kinased and run on a 20%acrylamide/7M Urea gel at 50C. I have never seen a
ladder extending in the n+1 direction before. How does this happen?
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