Sequencing length with ABI sequencer

J. David Spafford jspaffor at gpu.srv.ualberta.ca
Tue Jun 7 21:11:17 EST 1994


I use an ABI sequencer with a 9600 Perkin Elmers PCR machine.  Usually I can
get up to 400 bp of good sequence and no more.  ABI provides AmpliTaq with
their sequencing kit.  Would I get longer sequence if I spike it with Pfu
DNA polymerase?
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J. David Spafford
Department of Zoology, University of Alberta
jspaffor at gpu.srv.ualberta.ca
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