how to separate the nicked from of a plasmid from the closed form?

t_rolden at icrf.icnet.uk t_rolden at icrf.icnet.uk
Tue Jun 7 12:11:23 EST 1994


does anyone know a method to separate the nicked circular form of a plasmid
from the covalently closed circular form?. I have a mix which contain 50% of
each form, when I do a CsCl gradient to isolate the closed form I always have
contamination with the open form. does anyone konw a method to separare them,
different from the CsCl gradient?.

Many thanks,

T. Roldan




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