oligo deprotection

[Graham Threadgill]

Hi,

     My colleagues in our DNA synthesis department inform me that 
oligos can be cleaved and deprotected in 10 min using acetyl dC 
protected amidite.
     Cleavage takes 5 min at room temperature, deprotection 5 min at 
65c using a 1:1 ammonia : methylamine solution.
     The acetyl dC amidites are available from Beckman (800)742-2345 
for the Beckman Oligo 1000 synthesizer and from Glen Research (800) 
327-4536 for any other synthesizer.
     Hope this helps

Graham Threadgill
Beckman Instruments
2500 Harbor Blvd
Fullerton CA 92634

gjthreadgill at ccgate.dp.beckman.com

______________________________ Reply Separator _________________________________
Subject: Re: oligo deprotection
Author:  leach at mbcrr.harvard.edu (Martin Leach) at INTERNET
Date:    6/6/94 12:00 AM